Peraktikum Pembelahan Sel | Biologi Umum | UPT Laboratorium Terpadu UHO

Introduction

This tutorial provides a step-by-step guide to understanding the process of cell division, particularly focusing on the practical aspects demonstrated in the video "Peraktikum Pembelahan Sel" by UPT LAB. TERPADU UHO. Cell division is a fundamental biological process that is crucial for growth, development, and reproduction in living organisms. This guide aims to simplify the practical techniques involved, making them accessible for students and enthusiasts in biology.

Step 1: Preparing the Microscope

• Gather all necessary materials, including:
  • A microscope
  • Prepared slides of cells undergoing division
  • Staining solutions (if required)
• Ensure the microscope is clean and functioning properly.
• Adjust the light source for optimal visibility.
• Place the prepared slide on the microscope stage and secure it with stage clips.

Step 2: Observing Cell Division Phases

• Start with the lowest magnification to locate the cells.
• Gradually increase the magnification to observe the details.
• Focus on the different stages of cell division:
  • Interphase: Cells appear normal with a distinct nucleus.
  • Prophase: Chromosomes condense and become visible.
  • Metaphase: Chromosomes align at the cell equator.
  • Anaphase: Chromosomes are pulled apart to opposite poles.
  • Telophase: Two distinct nuclei form in the cell.

Step 3: Staining Cells for Better Visibility

• If the cells are not clearly visible, apply a staining solution:
  • Choose an appropriate stain (e.g., methylene blue).
  • Carefully add a drop of stain on the slide.
  • Allow the stain to sit for a few minutes to enhance contrast.
• Rinse the slide gently with distilled water to remove excess stain.

Step 4: Documenting Observations

• Use a notebook or digital device to record observations:
  • Note the characteristics of each phase.
  • Sketch or photograph the cells for reference.
• Pay attention to any anomalies or variations in the cell division process.

Step 5: Cleaning Up

• After completing the observation, clean the microscope lenses with lens paper.
• Dispose of any used slides and staining materials according to lab safety guidelines.
• Return all materials to their designated storage areas.

Conclusion

Understanding cell division is essential for studying biology, and this tutorial has outlined the necessary steps for observing this process in a laboratory setting. By following these steps, you can effectively prepare, observe, and document cell division. As a next step, consider exploring further topics such as the significance of mitosis and meiosis in different organisms or investigating the effects of various factors on cell division rates.