Percobaan Ingenhousz_Fotosintesis (Anabolisme) Biologi SMA Kls XII

Introduction

This tutorial outlines the Ingenhousz experiment, a crucial demonstration of photosynthesis. Understanding this experiment is essential for high school biology students, particularly those studying the processes of anabolism and metabolism. The experiment illustrates how light affects the photosynthetic activity of plants, showcasing the role of chlorophyll in this vital biological process.

Step 1: Gather Materials

To conduct the Ingenhousz experiment, you will need the following materials:

• A healthy green plant (preferably an aquatic plant like Elodea)
• A transparent container or glass beaker
• Water
• A light source (like a lamp)
• A stopwatch or timer
• Sodium bicarbonate (baking soda) to enhance carbon dioxide availability

Practical Tips

• Ensure the plant is healthy and well-oxygenated before starting.
• Use distilled water if available to minimize impurities.

Step 2: Prepare the Experiment Setup

1. Fill the container with water.
2. Add a small amount of sodium bicarbonate to the water to increase carbon dioxide levels.
3. Place the green plant in the container, ensuring it is submerged.
4. Position the light source about 30 cm above the container.

Common Pitfalls

• Avoid using too much sodium bicarbonate; a small pinch is sufficient.
• Ensure that the light source is stable and not obstructed during the experiment.

Step 3: Conduct the Experiment

1. Start the timer as soon as the light is turned on.
2. Observe the plant for signs of photosynthesis. Look for tiny bubbles forming on the leaves, which indicate oxygen production.
3. Record the time taken for the bubbles to appear and the rate at which they are produced.

Real-World Applications

• This experiment demonstrates the importance of light in photosynthesis, which is crucial for plant growth and oxygen production in ecosystems.

Step 4: Analyze the Results

• After conducting the experiment for a set period (e.g., 10-15 minutes), note the number of bubbles produced.
• Discuss how varying light intensity might affect the rate of photosynthesis.

Key Considerations

• Consider repeating the experiment with different light sources or distances to compare results.

Conclusion

The Ingenhousz experiment effectively illustrates how light impacts photosynthesis in plants. By following these steps, you can observe firsthand the relationship between light and the production of oxygen in plants. To further deepen your understanding, consider experimenting with different variables, such as light intensity and carbon dioxide levels, to explore their effects on photosynthesis.