PRAKTIKUM AWETAN BASAH

Introduction

In this tutorial, we will explore the process of preserving biological specimens, both animal and plant, which is essential for researchers and educators in the field of biology. This method, known as "awetan basah" or wet preservation, helps maintain the specimen's integrity for study and display. The steps outlined here will guide you through the necessary procedures for effective specimen preservation.

Step 1: Gather Necessary Materials

Before starting the preservation process, ensure you have all the required materials ready:

• Specimen (animal or plant)
• Formaldehyde or ethanol (as a preservative)
• Containers (glass jars or bottles with tight lids)
• Gloves (to protect your hands)
• Labels (for identifying specimens)
• Scissors or a scalpel (for size adjustments)
• Protective eyewear (to ensure safety)

Practical Advice

• Choose a preservative based on the type of specimen; formaldehyde is commonly used for animals, while ethanol is suitable for plants.
• Ensure that all containers are clean and dry before use to avoid contamination.

Step 2: Prepare the Specimen

Once you have your materials, prepare the specimen for preservation:

1. Examine the specimen to assess its condition.
2. If necessary, trim any excess parts using scissors or a scalpel.
3. Rinse the specimen gently with water to remove dirt or debris.

Practical Advice

• Handle the specimen carefully to avoid damage, especially if it is delicate.
• Make sure to wear gloves during this process to prevent contamination.

Step 3: Preservation Process

Now, it's time to preserve the specimen:

1. Place the specimen in the chosen container.
2. Pour the preservative (formaldehyde or ethanol) into the container until the specimen is completely submerged.
3. Seal the container tightly with a lid.

Common Pitfalls to Avoid

• Ensure the specimen is fully submerged to prevent decay.
• Use the appropriate concentration of preservative; for formaldehyde, a 10% solution is typical.

Step 4: Labeling the Specimen

Proper labeling is crucial for identification and future reference:

1. Create a label with the following information:
   • Scientific name
   • Common name
   • Date of preservation
   • Collector’s name
2. Attach the label securely to the container.

Practical Advice

• Use waterproof labels to prevent fading or damage over time.
• Keep a log or database of all specimens for easy reference.

Step 5: Storage of Preserved Specimens

Store your preserved specimens in a suitable environment:

• Keep the containers in a cool, dark place away from direct sunlight.
• Regularly check the specimens for any signs of leakage or degradation.

Practical Advice

• Monitor the level of preservative and refill as necessary to ensure complete coverage of the specimen.

Conclusion

Preserving biological specimens through the wet preservation method is a valuable skill for researchers. By following these steps—gathering materials, preparing the specimen, conducting the preservation, labeling, and proper storage—you can ensure that your specimens remain intact for future study and observation. For further exploration, consider researching different preservation techniques or expanding your collection with diverse specimens.