How to Build a Laboratory (for CHEAP)

Introduction

This tutorial provides a comprehensive guide on how to set up a laboratory for tissue culture without breaking the bank. You'll learn about essential equipment, supplies, and tips to create a functional lab space tailored to your needs.

Step 1: Understand the Lab Overview

• Familiarize yourself with the overall layout and functionality of your lab.
• Identify key areas: workspace for sterile operations, storage for supplies, and equipment stations.

Step 2: Acquire a Laminar Flow Hood

• The laminar flow hood is crucial for maintaining a sterile environment.
• Look for affordable options through public surplus sales or second-hand markets.
• Ensure it has a HEPA filter to effectively filter out contaminants.

Step 3: Source Essential Equipment

Magnetic Stirrer

• A magnetic stirrer is used for mixing solutions uniformly.
• Choose one that fits your budget, and check for used models online.

Orbital Shaker

• An orbital shaker helps in evenly shaking samples in culture flasks.
• Similar to the stirrer, consider purchasing second-hand to save money.

Bactizapper

• This device is used to sterilize surfaces and equipment quickly.
• Look for budget-friendly models or DIY options if you're handy.

Glass Bead Sterilizer

• Use a glass bead sterilizer for quick sterilization of tools.
• This is often a cost-effective alternative to traditional methods.

Alcohol Lamp

• Note that while alcohol lamps are common, they can be risky due to open flames.
• Consider safer alternatives for sterilization, like electric sterilizers.

Autoclaves and Pressure Cookers

• Autoclaves are essential for sterilizing media and tools.
• A pressure cooker can serve as a budget-friendly alternative for steam sterilization.

Instapot

• An Instapot can also be used for sterilization if it has a pressure function.
• This multifunctional appliance can save space and money.

Step 4: Optimize Lighting

• Good lighting is vital for plant growth and lab visibility.
• Look for LED grow lights that are energy efficient and effective.

Step 5: Stock the Laboratory

Essential Tools and Supplies

• Forceps, Scissors, and Trays: Essential for handling samples.
• Sterilization Pouches: Use these for safely storing sterile tools.
• pH Meter: Important for measuring the acidity of your media.
• Micropipettes: Necessary for precise liquid handling.
• Graduated Cylinders: Required for measuring volumes accurately.
• Scale: For weighing media and other materials.
• Media Bottles and Supplies: Stock up on the necessary media for your cultures.

Conclusion

Setting up a laboratory for tissue culture on a budget is entirely feasible with the right knowledge and resources. Start by acquiring essential equipment, optimize your space, and ensure you have a solid stock of supplies. Remember to research affordable options and consider second-hand purchases. Happy experimenting!