JUARA 2 MYRES 2022 || Bidang MST || MAN 2 Tasikmalaya || Nabilah Husniyyah

Introduction

This tutorial outlines the research project conducted by Nabilah Husniyyah from MAN 2 Tasikmalaya, which earned second place in the MYRES 2022 competition in the field of Mathematics, Science, and Development (MST). The focus of the study is on profiling and docking potential anti-cancer compounds derived from the right wing of flies through GC-MS characterization. This guide provides a step-by-step approach to conducting a similar research project.

Step 1: Define Your Research Objective

• Identify the specific problem you want to address. In this case, the objective was to explore potential anti-cancer compounds.
• Formulate a clear research question. For example, "What compounds from the right wing of flies exhibit anti-cancer properties?"

Step 2: Gather Background Information

• Conduct literature reviews to understand previous research on anti-cancer compounds and GC-MS characterization.
• Look for relevant studies on the biology of flies and the compounds they produce.
• Make notes on key findings that can inform your approach.

Step 3: Sample Collection

• Collect samples from the right wing of flies. Ensure that the collection method preserves the integrity of the samples.
• Use sterile tools and conditions to avoid contamination.

Step 4: Prepare Samples for GC-MS Analysis

• Extract compounds from the collected samples using appropriate solvents.
• Follow these steps:
  • Grind the wing samples in a suitable solvent.
  • Filter the solution to remove solid particles.
  • Concentrate the extract if necessary.

Step 5: Conduct GC-MS Characterization

• Run the prepared samples through Gas Chromatography-Mass Spectrometry (GC-MS).
• Ensure your equipment is calibrated and settings are optimized for detecting the compounds of interest.
• Record the data generated during the analysis.

Step 6: Data Analysis

• Analyze the GC-MS results to identify the compounds present.
• Compare the retention times and mass spectra with known databases to determine the identity of the compounds.
• Take notes on any compounds that show potential anti-cancer activity.

Step 7: Docking Studies

• Use molecular docking software to evaluate how the identified compounds interact with cancer-related proteins.
• Steps may include:
  • Selecting target proteins based on literature.
  • Preparing the compounds and proteins for docking.
  • Running the docking simulations and analyzing the binding affinities.

Step 8: Present Your Findings

• Compile your research into a clear presentation or report.
• Include sections such as:
  • Introduction
  • Methodology
  • Results and Discussion
  • Conclusion
  • References
• Prepare visual aids like charts or graphs to enhance understanding.

Conclusion

This tutorial provides a structured approach to conducting research similar to Nabilah Husniyyah's project on anti-cancer compounds. By following these steps, you can systematically explore and analyze biological samples, leading to valuable scientific insights. As a next step, consider applying this methodology to other biological samples or compounds to expand your research portfolio.