Praktikum Farmakologi Dasar : "Cara Menganestesi dan Mengorbankan Hewan Uji" | Percobaan 4

Introduction

This tutorial provides a step-by-step guide on how to anesthetize and euthanize test animals, specifically mice and rats, as demonstrated in the pharmacology practical from the video by Nur Aulia. Understanding proper techniques in handling laboratory animals is crucial for students in pharmacology and veterinary sciences, ensuring ethical practices and accuracy in research.

Step 1: Understanding Anesthesia

• Anesthesia is used to induce a reversible loss of sensation or consciousness in animals.
• It is essential for minimizing pain and distress during procedures.
• Common anesthetic agents for mice and rats include:
  • Isoflurane
  • Ketamine combined with Xylazine

Practical Tips

• Ensure you have a suitable anesthetic setup, including a vaporizer for inhalant anesthesia.
• Always monitor the animal's vital signs during the procedure.

Step 2: Preparing the Anesthesia Equipment

• Gather necessary equipment:
  • Anesthetic machine or vaporizer
  • Mask or induction chamber for the animal
  • Syringes and needles for injectable anesthetics
  • Oxygen supply if using inhalant anesthetics
• Ensure all equipment is clean and functioning properly.

Common Pitfalls

• Do not rush the setup; verify all connections and equipment before use.
• Ensure you have the correct dosage for the animal's weight.

Step 3: Anesthetizing the Animal

• Place the animal in the induction chamber or fit the mask securely.
• If using injectable anesthesia:
  • Calculate the correct dose based on the animal's weight.
  • Administer the anesthetic via an intramuscular or subcutaneous injection.
• Monitor the animal until it reaches the desired level of anesthesia.

Practical Advice

• Observe the animal for signs of adequate anesthesia, such as loss of reflexes and steady breathing.

Step 4: Conducting the Procedure

• Once the animal is adequately anesthetized, proceed with the necessary experimental procedure.
• Ensure that all actions are performed ethically and in accordance with institutional guidelines.

Step 5: Euthanizing the Animal

• Euthanasia should be performed humanely, following appropriate methods such as:
  • Overdose of anesthetic agents
  • Cervical dislocation (if trained and permitted)

Important Considerations

• Always comply with ethical standards and legal regulations regarding animal research.
• Document the procedure and any observations during the process.

Conclusion

This tutorial outlines the essential steps in anesthetizing and euthanizing test animals, emphasizing the importance of ethical practices in pharmacology. Always ensure that you are familiar with institutional guidelines and best practices for research involving animals. For further learning, consider exploring the pharmacology curriculum or ethical guidelines provided by veterinary associations.